Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts

Subcritical water extraction of horse chestnut (Aesculus hippocastanum) parts, such as seeds, seed shell, bark and leaves has been performed in a batch reactor at temperatures 150, 200 250?C times 5, 15 30 min. The obtained extracts were analyzed by spectrophotometric methods to determine the total phenols, carbohydrates antioxidant activity. Furthermore, compounds detected extracts, triterpene saponins (escins), coumarin glycosides (esculin fraxin), phenolic (chlorogenic, neochlorogenic gallic acids) furfurals (5-hydroxymethyfurfural, furfural, 5-methylfufrual) quantified using HPLC. High amount escins was while highest amounts esculin fraxin extracts. phenol content shell which implies that these gave